Metagenomic analyzes on Hexyon

Metagenomic analyzes on Hexyon

With these analyzes we came to the conclusion of the first level screening of the Hexyon vaccine


Focal points of the results

DNA analysis:

  • DNA from bacterial cultures used for the production of toxins (diphtheria, tetanus and pertussis) and Haemophilus influenzae B antigens: this DNA is immunogenic and is able to stimulate the formation of inflammatory cytokines and therefore contribute to a consistent inflammation both at the injection site and systemic. The question arises as to whether these fragments are potentially capable of causing autoimmune reactions and integrate into human DNA causing mutations. Since adjuvant aluminum is also present in the vaccine, these fragments are most likely linked to aluminum, which protects its degradation by enhancing its biological and toxicological effect, still largely unknown.

  • Monkey DNA Cercopithecidae: this DNA can be derived from Vero cells, i.e. immortalized. This DNA is found in traces and is degraded, so it should not be able to integrate itself into the host DNA. The fact remains that its presence is a proof of the incomplete reaction of formaldehyde and glutaraldehyde on the genetic material, which should instead be completely destroyed, and the presence of aluminum, as for the other genetic material, can make it stable to degradation over time. , expanding the potential toxic effects.

Adventitious Virus Analysis:

  • Phages: tetanus phage (Clostridium phage phiCT453A). It can potentially cause autoimmune diseases, especially if linked to aluminum.

  • Vectors used for cloning, including the SV40 vector: these are fragments of RNA that most likely come from the production process of the hepatitis B antigen NB: in this case SV40 is not the adventitious virus that was found in the attenuated polio vaccine, but a vector normally used for the genetic recombination for the production of engineered vaccines. The toxicology of these fragments is unknown but, if they are used to integrate fragments of genetic material, they could also integrate into the host DNA. Since the quantity is very small, the biological effect is not impossible to define.
    It remains the impossibility of defining the toxicity of this contamination linked to aluminum.

RNA analysis:

  • RNA from bacterial cultures used for the production of toxins (diphtheria, tetanus and pertussis) and Haemophilus influenzae B antigens: the above applies to DNA. In reality it is bacterial DNA and RNA partially degraded by the effect of formaldehyde, and therefore it is not possible from these data to understand if they are able to integrate into DNA, while it is very likely that they are capable of causing persistent inflammation and autoimmunity .

  • Monkey RNA: RNA may be able to form proteins, but these have not been detected in mass spectrometry (or because below the limit of nanograms, or because they are linked to aluminum and therefore cannot be sequenced and cannot be identified, or because this RNA is not working). As with bacterial RNA, it can bind to aluminum and cause autoimmunity and inflammation.

  • Poliovirus 1 and 2: they are supposed to be deactivated by formaldehyde and therefore not infectious, however the question always applies: aluminum alloys can be neurotoxic? In the technical sheet he writes that the power of poliovirus is defined by the effectiveness of the antigen D. The response received by EMA (always with reference to our first analyzes of August 2018) makes it clear that the genomes of the viruses should no longer be found.

Conclusions

Overall, this analysis tells us that compared to Infanrix hexa (the other hexavalent analyzed), the treatment with formaldehyde is much milder, and there is genetic material from the starting crops, which should not be there at all. This can pose a potential risk for autoimmunity, local and systemic inflammation, genetic mutations.


Bibliography

  • J Am Soc Nephrol. 2004 Dec; 15 (12): 3207-14. Short bacterial DNA fragments: detection in dialysate and induction of cytokines. Schindler R1, Beck W, Deppisch R, Aussieker M, Wilde A, Göhl H, Frei U.
  • PLoS Genet. 2013; 9 (10): e1003877. A review of bacteria-animal lateral gene transfer may inform our understanding of diseases like cancer. Robinson KM1, Sieber KB, Dunning Hotopp JC.
  • PLoS Comput Biol. 2013; 9 (6): e1003107. Bacteria-human somatic cell lateral gene transfer is enriched in cancer samples. Riley DR1, Sieber KB, Robinson KM, White JR, Ganesan A, Nourbakhsh S, Dunning Hotopp JC.
  • PLoS One. 2017 Aug 11; 12 (8): e0182909. Comparative pathogenomics of Clostridium tetani. Cohen JE1, Wang R2, Shen RF2, Wu WW2, Keller JE1.
  • Front Microbiol. 2018 Jun 27; 9: 1394. Beyond Bacteria: Bacteriophage-Eukaryotic Host Interactions Reveal Emerging Paradigms of Health and Disease. Chatterjee A1, Duerkop BA1.

Download: CORVELVA-Report-analysis-metagenomic-of-Hexyon.pdf